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. 2014 Jul 21;53(31):5150–5161. doi: 10.1021/bi5007404

Table 1. Primers Used for Site-Directed Mutagenesis.

mutant primers
T348Y Fwd 5′-GCGCCTATTGGGACTACGAGATCAAGCGCGCG-3′
  Rev 5′-CGCGCGCTTGATCTCGTAGTCCCAATAGGCGC-3′
S607Y Fwd 5′-AGACGCTCGACGATGCGCTCTATGAGCTGCGCG-3′
  Rev 5′-GAGCGCATCGTCGAGCGTCTTGCCGCCCTCG-3′
D778Y Fwd 5′-GCTGCCGGAGCAGGTCGCCTACGACGTTGTCACC-3′
  Rev 5′-GGCGACCTGCTCCGGCAGCGCGGTGGCATCG-3′
D779A Fwd 5′-TGCCGGAGCAGGTCGCCGACGCCGTTGTCACCTCC-3′
  Rev 5′-GTCGGCGACCTGCTCCGGCAGCGCGGTGGC-3′
D779W Fwd 5′-TGCCGGAGCAGGTCGCCGACTGGGTTGTCACCTCC-3′
  Rev 5′-GTCGGCGACCTGCTCCGGCAGCGCGGTGGC-3′
D779Y Fwd 5′-CCGGAGCAGGTCGCCGACTACGTTGTCACCTCCGC-3′
  Rev 5′-GCGGAGGTGACAACGTAGTCGGCGACCTGCTCCGG-3′