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. 2014 Jul 16;53(31):5080–5091. doi: 10.1021/bi5003794

Table 8. Characteristics of P450s Lacking the Conserved Phenylalanine.

        other modifications
    
P450 Phe replacement activity active intermediate compound insert on proximal surface mutate I helix motif surface charge modification vs P450 2B4 refs
CYP74A (AOS), allene oxide synthase Trp unsaturated fatty acid hydroperoxide alkyl dehydratase II +9 amino acids yes yes (21), (22)
CYP74B (HPL), hydroperoxide lyase Trp unsaturated fatty acid alkyl hydroperoxide isomerase II +9 amino acids yes yes (21)
CYP74D (DES), divinyl ether synthase Trp unsaturated fatty acid alkyl hydroperoxide dehydratase II +9 amino acids yes yes (21)
CYP8A1, prostacyclin synthase Trp unsaturated fatty acid endoperoxide isomerase II +12 amino acids yes yes (23)
CYP176A1 P450cin Leu monooxygenation of 1,8-cineole I no yes no (19)
CYP152A1, P450 BSβ Gln fatty acid α and β C-hydroxylation I +4 amino acids yes yes (70)
CYP152B1, P450 SPα Gln fatty acid α C-hydroxylation I +4 amino acids yes yes (69)