Figure 4. Transcriptional regulation of endogenous target genes by PML/RARA mutants.
(A-B) NIH-3T3 cells stably expressing the indicated PML/RARA mutants were treated or not with 10−6 M ATRA overnight. RARB and Tg II gene expression were analyzed by quantitative RT-PCR. (C-G) The indicated mutant PML/RARA-transduced primary hematopoietic progenitors were treated with or without 10−6 M ATRA for 14 hours. RARB, Tg II, HCK, PU.1 and ID1 gene expression was also analyzed by quantitative real-time PCR. All results from quantitative real-time PCR were normalized to β-actin. Results, obtained from three independent experiments, are given as mean ±standard deviations.