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. 2014 Aug 13;9(8):e104885. doi: 10.1371/journal.pone.0104885

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© 2014 Posnien et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A Transcript length distribution of different assemblies. Illumina-based assemblies result in more long transcripts compared to the 454/EST assembly. B The 454/EST assembly results in fewer transcripts and fewer unique transcripts based on BLASTX searches against the nr database compared to Illumina-based assemblies (left y-axis). The combination of datasets results in improved NXX (N25, N50, N75) values for the respective assemblies (right y-axis). Note that Assemblies 1 and 2 are the intermediate assemblies of the stepwise assembly strategy and Assembly 3 is the final assembly including unsupported 454/EST transcripts (see Materials and Methods for details).