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. 2014 Aug 13;9(8):e104896. doi: 10.1371/journal.pone.0104896

Figure 4. mRNA expression levels in Brown and White Adipose Tissue, Cytochrome C Oxidase and Citrate Synthase Activities and Mitochondrial DNA content in White Adipose Tissue of F1 mice.

Figure 4

A, B) mRNA expression levels of genes encoding proteins involved in the mitochondrial function in (A) Brown Adipose Tissue (BAT) and (B) White Adipose Tissue (WAT) from 7-months-old F1-CD and F1-LPD males mice. mRNA relative levels are normalized relatively to Nono expression level and expressed as fold change relative to the control value measured in F1-CD. Values are means ± sem for at least 8 mice/group. **p≤0.05, *p≤0.08. C) Cytochrome C Oxidase (COX  =  Complex IV) and Citrate Synthase (CS) activities are measured in WAT from 7-months-old F1-CD and F1-LPD males mice and expressed as a percentage of the COX/CS ratio of F1-CD mice. Values are means ± sem for at least 8 mice/group. D) Mitochondrial density is estimated by measuring the mitochondria DNA (mtDNA) content relatively to the nuclear DNA (nuclDNA) content in White Adipose Tissue. mtDNA/nuclDNA ratio was calculated as the ratio of COX1 to cyclophilin A DNA levels, determined by real-time PCR, in DNA extracted from White Adipose Tissue of 7-months-old F1-CD and F1-LPD males mice. Results were normalized by the mean value of the control condition set to 1 unit. *p≤0.05.