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. 2014 Aug 13;9(8):e104970. doi: 10.1371/journal.pone.0104970

Figure 7. Calumenin trafficking in CFBE41o- cells expressing wild-type CFTR.

Figure 7

Calumenin or CFTR (green) in CFBE41o- wild-type or F508del cells were visualized using fluorescence microscopy. Second labeling was performed for either PDI (an ER marker). Golgi marker or EEA1 (marker for endosomal vesicles) (red). The nuclei were stained blue with DAPI. Colocalisation of green and red pixels was detected in merged images (yellow). A. Calumenin and PDI (for ER staining) B. Calumenin and Golgi and C. Calumenin and EEA1 in CFBE41o- wild-type cells. D. CFTR and EEA1 in CFBE41o- wild-type cells. Scale bar: 20 µm.