| mM | g/ltr | |
| 135 | NaCl | 7.8894 |
| 5.4 | KCl | 0.40257 |
| 1.8 | CaCl2 | 0.26464 |
| 1 | MgCl2 | 0.2033 |
| 5 | HEPES | 1.1915 |
| Adjust pH to 7.2 with NaOH | ||
| Add 20 mg/ml-1 biocytin |
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