| 1 drop | Reagent A |
| 1 drop | Reagent B |
| 0.5 ml | 10% Triton X100 |
| Dissolve in 9.5 ml 0.05M PB (45 min in advance) | |
| Add 410 µl solution/well | |
| Protocol for ABC-solution for one 24-well plate. |
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