6 g	Analytical grade glycerol
2.4 g	Mowiol 4-88
Manually stir 10 min to coat Mowiol with glycerol
6 ml	Distilled H2O
Incubate at RT for 2 hr
12 ml	0.2 M Tris pH 8.5
Incubate in 50 °C waterbath for 1hr - O/N, stir occasionally
Centrifuge 15 min at 5,000 x g, aliquot and store at -20 °C