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. 2014 Aug 13;9(8):e105118. doi: 10.1371/journal.pone.0105118

Figure 5. Attenuation of 6-OHDA-induced cell death by Lv-PSPN-MSC in MES23.5 cells.

Figure 5

(A) Effect of different concentrations of 6-OHDA (1–200 µM) on metabolic rate. (B) The apoptotic rates of the MES23.5 cells treated in (C, D, E, F) were assessed at the indicated time periods by annexin V/7-AAD staining. (C) MES23.5. (D) MES23.5+6-OHDA. (E) 6-OHDA+MES23.5+MSCs. (F) 6-OHDA+MES23.5+ Lv-PSPN-MSCs. Cells were analyzed for annexin V/7-AAD staining by flow cytometry. Events in each of the four quadrants are as follows: Lower left, viable cells; lower right, cells in the early-to mid-stages of apoptosis; upper right, cells in the late-stages of apoptosis; upper left, mostly nuclear debris.*P<0.05.