Fig. 2.

CRE-SPAP production in response to cimaterol in the absence and presence of CGP12177 in stable cell lines expressing (A) β₁-WT, (B) β₁-TM4, and (C) β₂-WT. Bars represent basal CRE-SPAP production, that in response to 10 µM isoprenaline or that in response to 1–30 nM CGP12177 alone. Data points are mean ± S.E.M. of triplicate determinations. These single experiments are representative of (A) six, (B) eight, and (C) nine separate experiments. This figure shows that CGP12177 inhibits cimaterol responses with similar high affinity at β₁-WT, β₁-TM4, and β₂-WT.