Figure 2.

HCY increases the apoptosis of neurons and suppresses the expression of Camk2b. A, B: The results of Tunel Assay for neurons in normal and Hhcy groups. Note that the neurons with cytoplasm in red (TUNNEL +) which indicates apoptotic neurons were obviously more in Hhcy group than those in normal group, with the percentage of apoptotic neurons much higher in Hhcy group. C: The result of RT-PCR for the CamK2b mRNA in both normal and Hhcy groups. Compared with normal group, the transcription level of Camk2b mRNA in neurons exposed to Hhcy was obviously lower. D: CamK2b protein in both normal and Hhcy groups were measured by Western Blot which showed that the expression of CamK2b protein in Hhcy group was suppressed. E: CamK2b protein relative expression levels based on the results of Western Blot. ##P < 0.01 VS normal group.