Figure 3.

Camk2b protects hippocampal neurons from HCY-induced apoptosis. A: Result of RT-PCR for CamK2b mRNA in neurons transfected with CamK2b (Camk2b group) or empty plasmid (control group), which showed a higher transcription level of CamK2b mRNA in Camk2b group than that in control group. B: Result of Western Blot for Camk2b protein in both Camk2b (the right column) and control (the left column) groups, which showed the overexpression of Camk2b protein in Camk2b group. C: Camk2b protein relative expression levels based on the results of Western Blot. D, E: The results of Tunnel Assay for the apoptosis of hippocampal neurons in both Camk2b and control groups, which showed less apoptotic neurons with cytoplasm in red (TUNEL +) and lower percentage of apoptotic neurons in Camk2b group than that in control group. F: The results of MTT Assay for the activity of neurons in both Camk2b and control groups, which showed that neurons in Camk2b group had higher activity than those in control group. #P < 0.05, ##P < 0.01 VS control group.