Figure 5.

The expression levels of HIF-1α related co-activator and downstream elements were elevated after the transfection of CamK2b. A: The mRNA relative expression levels of Bcl2, Camk2b, eNOS, EP300, EPO, HIF-1α, VEGF and P21 in four groups (Normal: neurons without exposure to Hhcy, HHcy: neurons exposed to HHcy, Camk2b + HHcy: neurons exposed to HHcy and transfected with Camk2b, Control + HHcy: neurons exposed to HHcy and transfected with empty plasmid) measured by RT-PCR. Note that the transcription levels of Bcl2, eNOS, EP300 and EPO were all the highest in Camk2b group than other three groups. B: The expression levels of the corresponding proteins in four groups measured by Western Blot, which showed that the expression levels of EP300, eNOS, Bcl2 and EPO were all higher in Camk2b group than those in HHcy and control groups.