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. 2014 Jul 7;15:224. doi: 10.1186/1471-2474-15-224

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Copyright © 2014 Nagai et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Morphological analysis and myofiber CSA measurements (μm²) of the medial gastrocnemius muscle. (I) The CSAs of the medial gastrocnemius muscle from the control, sham-operated, and immobilized knees after 1 and 16 weeks. (A, D) Control group. (B, E) Sham-operated knees. (C, F) Immobilized knees. Scale bars represent 100 μm (microscope magnification: ×400). (II-a, b) Measurement of the CSA (μm²) of the medial gastrocnemius muscle (only one displayed). Values are presented as mean ± SD; ^**P < 0.001. The CSA in the immobilized knees was lower than that of the control or sham knees at both time points.