Table 2.

Polymerase chain reaction protocol for GRP58, HPRT, and β-actin

Step	Temperature (°C)	Time	Number of cycles
Initial denaturation	95	5 minutes	1
Denaturation	95	20 seconds	40
Annealing*	58.3	30 seconds	40
Extension	70	5 seconds	40

Note:

^*60°C for annealing for GRP58 and β-actin, and 58.3°C for HPRT.