Table 2.
Mutation energies associated with the design of construct V5.
| Mutation | Location | Mutation energy (kcal/mol) in m3F8 Fab | Mutation energy (kcal/mol) in hu3F8 Fab | Resulting phenotype |
|---|---|---|---|---|
| LC: K24R | CDR L1 | −0.21 | −0.17 | Human |
| LC: S56T | CDR L2 | −0.05 | +0.04 | Human |
| LC: V58I | Framework | −0.64 | +0.01 | Human |
| HC: I20L | Framework | +0.43 | −0.86 | Human |
| HC: A62S | CDR H2 | −0.24 | −0.50 | Human |
| HC: F63V | CDR H2 | +1.91 | +1.80 | Human |
| HC: M64K | CDR H2 | +0.10 | −0.23 | Human |
| HC: S65G | CDR H2 | +0.82 | +1.10 | Human |
| HC: M89V | Framework | −0.50 | −0.29 | Human |
| Net result | +1.62 | +0.90 |