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Location of E507K and blood-resistant mutations in Taq crystal structure. “Fitness mutations” G59W, V155I, L245M, and F749I reside in the 5′–3′ exonuclease and Fingers domains, respectively, while fast-cycling mutation E507K is in the thumb domain (A) (Kim et al., 1995). E507 resides in close proximity to the primer portion of the primer-template duplex (B) (Li et al., 1998).
