Figure 5.

Amplification directly from blood. A 322 bp IGF target was amplified from 5 to 25 μl EDTA-blood using 50 ng of each mutant Taq DNA polymerase. Reactions were cycled on the SureCycler 8800 using the following parameters: 5 min at 90°C, followed by 30 cycles of 30 s at 95°C, 30 s at 60°C, 60 s at 72°C. Wild-type Taq can amplify the 322 bp from human genomic DNA in the absence of blood, but not in the presence of 1% blood (data not shown), even though the amount of EDTA introduced with 0.5 μl EDTA-blood (0.089 mM EDTA) is well below inhibitory levels.