Table 2.
Kinetic parameters of wild-type and mutant Taq polymerases.
| Enzyme | Extension rate (nt/s) | Processivity (nt) | Kd [DNA] (nM) |
|---|---|---|---|
| Taq (wt) | 50 | 20 | 102 |
| Taq 42 | 85 | 20 | 2.75 |
| Taq 1C2 | ND | ND | 1.9 |
| E507K | 85 | 20 | 1.1 |
| G59W | ND | ND | 91.6 |
ND, Not Determined.
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