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. 2014 Aug 14;4:110. doi: 10.3389/fcimb.2014.00110

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Copyright © 2014 Meißner, Eckelt, Basler, Meens, Heinzmann, Suwandi, Oelemann, Trenkamp, Holst, Weiss, Bunk, Spröer, Gerlach and Goethe.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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GC/LC-MS analysis of metabolites (A) and HPTLC analysis of lipids (B,C) from MAPwt and MAPΔmptD. (A) The heat map illustrates the significant concentration changes (overall results of three biological replicates). The color intensity and tones represent magnitude and direction of metabolic changes, respectively, with the magnitude of change ranging from white (reference) to red (positive deviation to reference) or blue (negative deviation to reference). (B,C) HPTLC analysis of polarity-fractionated lipids. Mobile phase in (B) chloroform/methanol 18:2 (v/v); in (C) chloroform/methanol/water 65:25:4 (v/v/v). Lane 1: crude lipids from MAPΔmptD; lanes 2, 4, 6 and 8: fractions of MAPwt; lanes 3, 5, 7, and 9: fractions of MAPΔmptD; lanes 2 and 3: chloroform fraction; lanes 4 and 5: acetone fraction; lanes 6 and 7: methanol fraction; lanes 8 and 9: chloroform/methanol 1:1 (v/v) column wash; lane 10: TDM standard; Lane 11: PIM standard (see text) yellow arrows = additional bands; yellow boxes = same bands with different intensities.