Figure 2.

Vit C resulted in the dysregulation of SIRT1, p53, and FOXO3 during oxidative stress in ARPE-19 cells. ARPE-19 cells were treated with indicated methods. The expression of SIRT1 (a), p53 (b), and FOXO3 (c) mRNA was detected by qRT-PCR. Ct values were normalized by 2^−ΔΔCt method as relative quantification. Bars represented mean ± SD of three independent experiments. ANOVA was performed to analyze the differences statistically. Statistical significance was expressed as *P < 0.05 and **P < 0.01.