Figure 3.

SIRT1 changed the effects of Vit C on viability, apoptosis, and intracellular ROS of ARPE-19 cells induced by H₂O₂. ARPE-19 cells were preincubated with RSV (10 mM) or NA (5 mM) for 4 h. Then, cells were exposed to H₂O₂ for 12 h after treatments of indicated concentration of Vit C. Viability (a), apoptosis (b), and intracellular ROS (c) were measured and compared among different groups. N1, N2, N3, and N4 were standing for the cells treated with H₂O₂ and without Vit C preincubation, cells treated with H₂O₂ and 20 μM Vit C, cells treated with H₂O₂ and 100 μM Vit C, and cells treated with H₂O₂ and 500 μM Vit C, respectively. Single treatment was performed in triplicate. Statistical significance was expressed as *P < 0.05 and **P < 0.01.