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. 2014 Aug 15;127(16):3463–3476. doi: 10.1242/jcs.146696

Fig. 4.

Fig. 4.

The knockdown of Rassf5 interferes with the polarization of neurons. (A–C) E14.5 brains were transfected by ex vivo electroporation with empty pCAGGS-U6 (control) or pCAGGS-U6-Rassf5 with an shRNA against Rassf5 (Rassf5 RNAi), and live-cell imaging of slice cultures was performed at 36 h after electroporation. (A) Representative images (maximum-intensity projection) of cortical slices at the indicated time-points are shown. CP, cortical plate; IZ, intermediate zone; VZ, ventricular zone. Scale bars: 50 µm. (B) Representative images (maximum-intensity projection) of transfected neurons after 23 h of live-cell imaging from control and Rassf5-knockdown slices are shown. Open arrowheads mark leading processes, black arrowheads mark trailing axons or processes of unpolarized neurons after knockdown of Rassf5. Scale bars: 10 µm. (C) The percentage of GFP-positive cells in the ventricular and subventricular zone (VZ), intermediate zone and cortical plate at 0, 6, 12 and 23 h is shown. Values are the mean±s.e.m. (three experiments, n = 200–400 neurons per time-point); ****P<0.0001 compared with control; two-way ANOVA.