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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Mol Microbiol. 2014 Jul 8;93(4):629–644. doi: 10.1111/mmi.12676

Figure 1. IscA has a unique copper binding activity among the iron-sulfur cluster assembly proteins.

Figure 1

Each protein encoded by the gene cluster iscSUA-hscBA-fdx was expressed in the E. coli copA/cueO/cusA mutant cells grown in LB media supplemented with or without 200 μM CuSO4. Proteins were purified from the cells and subjected to UV-visible absorption measurements. A), IscS; B), IscU; C), IscA; D), HscB; E), HscA; F), Ferredoxin. In each panel: spectrum 1, without CuSO4 in LB media; spectrum 2, with CuSO4 in LB media. Insert in each panel is a photograph of SDS-PAGE gel of purified proteins. The results are representatives of three independent protein preparations.