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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Mol Microbiol. 2014 Jul 16;93(4):759–775. doi: 10.1111/mmi.12693

Fig. 5.

Fig. 5

Transcription from the irp2 promoter is affected by Zn2+. The β-galactosidase activities from the irp2∷lacZ transcriptional reporter carried by KIM6+ (Znu +) or KIM6-2077+ (Znu -) are averages (with standard deviations) of replicate samples from two independent cultures. Strains were grown in cPMH2 at 37°C unsupplemented or with ZnCl2 or FeCl3 added to 10 μM. Statistical significances were calculated using the Student's two tailed t-test; p = <0.001 - ***. As previously demonstrated (Perry et al., 2003a), transcription from the irp2∷lacZ reporter in both strains was repressed by FeCl3 supplementation (p = <3 × 10-7; not shown on the graph for clarity).