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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Mol Microbiol. 2014 Jul 16;93(4):759–775. doi: 10.1111/mmi.12693

Fig. 7.

Fig. 7

A YbtQX- Znu- mutant (open symbols) has a severe growth defect and requires additional Zn2+ supplementation to restore growth, compared to single Znu- and YbtQX- mutants (closed symbols). Cells were grown at 37°C in cPMH2 with 1.0 μM FeCl3 and increasing levels of ZnCl2. Strains: KIM6-2066 (YbtQX- [ΔybtQX]); KIM6-2077+ (Znu- [ΔznuBC]); KIM6-2077.13 (YbtQX- [ΔybtQX] Znu- [ΔznuBC]). YbtQ, but not YbtX, is part of the IM ABC transporter (YbtPQ) required for the use of Fe3+ from Ybt (Perry and Fetherston, 2011). Growth curves shown are from one experiment that is representative of two or more independent experiments.