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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Mol Microbiol. 2014 Jul 16;93(4):759–775. doi: 10.1111/mmi.12693

Fig. 8.

Fig. 8

YbtX is required for Ybt-dependent Zn2+ uptake. Cells were grown at 37°C in cPMH2 with 1.0 μM FeCl3 and 0.6 μM ZnCl2. A. The growth of KIM6-2077.13 (YbtQX- [ΔybtQX] Znu- [ΔznuBC] is restored when carrying pYbtPQX, which expresses ybtPQX+ (YbtQX-/YbtPQX+) but not when carrying pYbtPQ, which expresses only ybtPQ+ (YbtQX-/YbtPQ+). B. A plasmid (pYbtX) expressing only ybtX+ restored the growth of KIM6-2077.13. KIM6-2077+ (Ybt+ Znu- [ΔznuBC]) and strains carrying the vector plasmid pACYC184 were used as controls. Growth curves shown are from one experiment that is representative of two or more independent experiments.