Figure 3.

Foxj2 is a direct target of miR-294—(a) The ‘FOX{I1,J2}’ motif shows a negative change in activity in the presence of miR-294. (b) Foxj2 mRNA log₂ fold changes (±1.96*SEM; n = 3) in the Melton et al. Dgcr8^{−/ −} versus miR-294 transfection (yellow), Sinkkonen et al. Dicer^{−/ −} versus miR-290-295 cluster transfection (dark brown) and Dicer^{−/ −} versus Dicer^{+/ −} (light brown) data sets, as well as the prediction scores for these genes as targets of miR-294 as given by ElMMo (30) (dark red) and TargetScan (aggregate P_CT) (4) (light red). (c) A luciferase reporter construct carrying the 3′UTR of Foxj2 is downregulated upon co-transfection with miR-294 relative to a construct carrying the Foxj2 3′UTR but with a mutated miR-294 target site (n = 9).