Table 1. KD values obtained with SPR in TBS.
| tel | tel | ILPR | c-MYC | |
|---|---|---|---|---|
| DARPin variant | KD from kinetics (nM) | KD from competition (nM) | KD from kinetics (nM) | KD from kinetics (nM) |
| 1C6 | 16 ± 3 | 56 ± 3 | nb | nb |
| 1C7 | 37 ± 15 | 110 ± 10 | 33 ± 8 | 81 ± 38 |
| 1C11 | 53 ± 22 | 100 ± 5 | 43 ± 29 | 49 ± 27 |
| 1G2 | 53 ± 44 | 69 ± 7 | 27 ± 9 | 43 ± 19 |
| 1G11 | 72 ± 46 | a | 44 ± 30 | 19 ± 3 |
| 1H4 | 62 ± 34 | 81 ± 7 | nb | nb |
| 2C10 | 48 ± 12 | 67 ± 5 | nb | nb |
| 2D10 | 34 ± 16 | 110 ± 10 | nb | nb |
| 2E4 | nb | nb | nb | 64 ± 40 |
| 2G7 | 20 ± 3 | 32 ± 2 | nb | nb |
| 2G10 | nb | nb | nb | 90 ± 59 |
nb, no binding, i.e. no or very weak RU signal.
aComplex behavior, could not be determined, see text.