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. 2014 Jul 16;42(14):9262–9269. doi: 10.1093/nar/gku592

Figure 4.

Figure 4.

Cleavage of DNA substrate by F-8 under different reaction conditions. All reactions were conducted using trace amounts of 5’-32P-labeled substrates (about 10 nM) with 1 μM deoxyribozyme F-8 in 50 mM HEPES or other appropriate buffer containing 400 mM NaCl, 100 mM KCl and 10 mM MgCl2. (A) Reactions were performed under standard conditions (pH 7.4) at different temperatures. (B) Reactions were performed at 37°C at different pHs. (C) Reactions were performed at 37°C and at pH 7.4 in the presence of different Cu2+ concentrations. (D) Reactions were performed at 37°C and at pH 7.4 in the presence of different Mn2+ concentrations.