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. 2014 Jul 16;42(14):9484–9492. doi: 10.1093/nar/gku593

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — Engineering and characterization of the fixed-gain amplifiers Amp32^C and Amp30^C. (A) The fixed-gain transcriptional amplifier comprises two terminals corresponding to the signal input and signal output. Here two amplifiers with different gains, Amp32^C and Amp30^C, are designed by using two different RBS sequences ahead of the hrpS gene. An arsenic responsive sensor is the input signal and gfp the output. (B) Steady state responses of the arsenic sensor without amplification and with amplification by Amp32^C and Amp30^C. The cells are induced by 12 varying concentrations of arsenite (0, 0.125, 0.25, 0.35, 0.5, 0.75, 1.0, 2.0, 4.0, 8.0, 16, 32 μM NaAsO₂). (C) The scatter plot shows the linear relationships between the non-saturated transcriptional inputs (the signal inputs that do not lead to maximum output level of the device) and the amplified outputs of Amp32^C and Amp30^C by fitting to a linear function. (D) Device noise characteristics of Amp32^C and Amp30^C. (E) Performance characteristics summary of the two amplifiers. Data shown in these figures are means and s.d. for three replicates performed on different days.