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. 2014 Jul 22;42(14):9195–9208. doi: 10.1093/nar/gku594

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© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Structural characterization of vesicles as exosomes. Characterization of vesicles released from neurites following potassium-induced depolarization. (a) Glutaraldehyde-fixed samples were sliced and photographed using a JEOL-1200EX transmission electron microscope. Scale bar = 200 nm for main photo, 100 nm for inset. Analysis of particle size using ImageJ software found the average diameter of these vesicles to be 106 ± 7.62 nm. (b) Western blot analysis of exosome markers LAMP1 and (c) Flot1. Each experiment was replicated 3 times and representative blots are depicted.