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(A) Readthrough efficiency of dual luciferase constructs with 3′ nested deletions of OPRK1, OPRL1, MAPK10 and AQP4. (B) 5′ and 3′ sequences of OPRL1 and OPRK1 swapped and readthrough efficiencies determined. (C) Analysis of the effect of sequences encoding the nascent peptide on the readthrough efficiency of OPRL1. Amino acids in gray-type face are encoded within the plasmid (C-terminus of Renilla) and those in bold type face are from OPRL1.
