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. 2014 Jul 10;42(14):8928–8938. doi: 10.1093/nar/gku608

Figure 4.

Figure 4.

Systematic mutational analysis of the 13 nucleotides immediately 3′ of OPRL1 TGA (A) and OPRL1-CARYYA TGA (B). The dotted lines represent the mean readthrough efficiencies of the wild-type OPRL1 (A) and OPRL1-CARYYA (B). Sequences at the bottom of each graph are those of the 13 nucleotides immediately 3′ of OPRL1 TGA (A) and OPRL1-CARYYA TGA (B). The triplet of nucleotides immediately above each nucleotide of these sequences represent the substitution mutations (those in B introducing a zero frame stop codon were excluded).