Figure 5.

Concordance between RASL-seq and RNA-seq measurements. Control B cell lysates were split for parallel analysis by either RASL-seq or RNA-seq global transcriptional profiling. (A) RNA-seq read counts generated from cells treated with CD40L and IL-4 for 7 days were normalized to transcript length and plotted against RASL-seq read counts from the corresponding probe sets. When there were multiple RASL probe sets targeting the same transcript, data from the probe set reporting the highest signal was used. Splice junction probe sets were excluded from this analysis. (B) IL-4 induced fold changes in gene expression were measured independently by RNA-seq and RASL-seq. The results are compared so as to assess their correlation.