Figure 1.

Subnuclear localization of topo IIβ under different conditions. Topo IIβ and nucleolin (as a nucleolus marker) were immunostained with cross-reactive antibodies in human (HEK293), rat (Rat-1) and mouse (Neuro 2a) cell lines under four different conditions. Topo IIβ (green) and nucleolin (magenta) are shown together with merged images and DNA (DAPI). Scale bars, 5 μm. (A) Cells were fixed with PFA at 37°C. (B) Cells were fixed with PFA at 0°C. (C) Cells were first treated with 30 μM ICRF-193 for 15 min and then fixed at 37°C. (D) Cells were first treated with 10 mM sodium azide and 6 mM 2-deoxy-d-glucose for 40 min and then fixed at 37°C.