Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jul 17;42(14):9005–9020. doi: 10.1093/nar/gku640

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2014. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 4. — Migratory behavior of functionally defective topo IIβ mutants expressed in nucleus that are tagged with EGFP at C-terminus. (A) Summary of amino acid changes introduced to WT topo IIβ. (B) Assessment of in vivo topo IIβ activity by the band depletion assay. Activities for endogenous enzyme (labeled topo IIβ) and EGFP-fused exogenous one (topo IIβ-EGFP) can be discriminated by the size difference. EGFP-fused mutants are abbreviated: GI, G173I; LF, L178F; YS, Y814S. (C) Changes in the nuclear distribution of topo IIβ mutants after ICRF-193 treatment. Shown here are EGFP fluorescence images (upper panel; scale bar, 5 μm) and the ratio of nucleolar fluorescence to whole nuclear fluorescence (lower graph). The ratio was determined by using the ‘threshold’ and the ‘integrated density (IntDen)’ functions of ImageJ software. Bars, mean/SD (n = 50).