Figure 4.

Discrimination between trans-spliced and circular RNAs for the six confirmed PtNcl RNA events. (A) RT-PCR results and (B) expression fold changes (as determined by qRT-PCR) for the six PtNcl RNA events in the indicated human ESC lines, before and after RNase R treatment. (C) Comparisons of the expression fold changes for the six PtNcl RNA events in poly-A tailed RNAs (purified mRNAs) and poly-A tailed RNAs treated with RNase R in the indicated human ESC lines. Error bars represent the mean ± standard deviation. P-values were estimated by the two-sample, two-tailed t-test. Significance: ***P < 0.001. NS: not significant.