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. 2014 Jul 23;42(14):9424–9435. doi: 10.1093/nar/gku656

Figure 5.

Figure 5.

Sponge inhibitors of miR-9 and isomiR-9. (A) Outline of sponge constructs pcDNA-miR-9 amd pcDNA-isomiR-9 (see Materials and Methods). HEK293 cells were transfected with the indicated concentrations of each sponge vector with either (B) pGL3-CDH1–3′UTR (400 ng) and miR-9 (12 nM) or (C) pGL3-DNMT3B-3′UTR (400 ng) and isomiR-9 (12 nM). All results were normalized by renilla luciferase. For sponge sequences see Supplementary Table S4. Error bars represent the standard deviation obtained from three independent experiments, * represents statistical significance (between sponges) at P < 0.05.