Table 2. Summary of the luciferase assay results for the targeting of the indicated 3′UTRs by the indicated miRNAs and isomiRs. The brackets mark targetscan predictions that are discordant with the results.
| No | mRNA | MiRNA | Prediction | Luc assay | Notes |
|---|---|---|---|---|---|
| 1 | PTEN | miR-367 | √ | √ | New target |
| 3′ isomiR-367 | √ | √ | |||
| 2 | BTG2 | miR-367 | (√) | X | |
| 3′isomiR-367 | (√) | X | |||
| 3 | CDH1 | miR-9 | √ | √ | Confirmation (ref) |
| 5′ isomiR-9 | X | X | (Ma et al., 2010) | ||
| 4 | DNMT3B | miR-9 | X | X | New target |
| 5′ isomiR-9 | √ | √ | |||
| 5 | NCAM2 | miR-9 | X | X | New target |
| 5′ isomiR-9 | √ | √ | |||
| 6 | HMGA2 | miR-9 | √ | √ | New target |
| 5′ isomiR-9 | (X) | √ | |||
| 7 | BTG1 | miR-302a | √ | √ | New target |
| 5′ isomiR-302a | (X) | √ | |||
| 8 | Rock1 | miR-302a | X | nt | New target |
| 5′ isomiR-302a | (√) | X |