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. 2014 Jun 30;289(33):22589–22599. doi: 10.1074/jbc.M114.560979

FIGURE 3.

FIGURE 3.

The RPR domain of CREPT is responsible for TCF4. A, a graphic representation of the CREPT protein structure. CREPT consisted of the RPR and CCT domains. B, both RPR and CCT domains interact with β-catenin. FLAG-β-catenin and Myc-CREPT full-length and RPR and CCT domains were co-expressed in HEK293T cells. The cell lysates were incubated with anti-Myc antibody for IP assay. C, the RPR domain of CREPT interacts with HA-TCF4. HA-TCF4 and Myc-CREPT full-length and RPR and CCT domains were co-expressed in HEK293T cells. Cell lysates were incubated with anti-Myc antibody for IP experiment. D, the RPR domain is critical for promoting the Wnt signaling of CREPT. LEF-1-luciferase and pRL-TK reporters were co-expressed with Myc-CREPT full-length, RPR and CCT domains as well as Wnt1. Luciferase activity was detected after 24 h transfection.