Skip to main content
. 2014 Jun 30;289(33):22600–22613. doi: 10.1074/jbc.M114.547380

FIGURE 6.

FIGURE 6.

miR-146b regulates the PDGFRA-JNK-JUN-GATA-1 pathways in erythroid and megakaryocytic differentiation. A, immunoblot analysis of PDGFRA, JNK, and JUN levels; phosphorylated PDGFRA, JNK, and JUN levels; and HEY1 and GATA-1 levels during hemin-induced erythroid differentiation of K562 cells. B, immunoblot analysis of PDGFRA, JNK, and JUN levels; phosphorylated PDGFRA, JNK, and JUN levels; and HEY1 and GATA-1 levels during PMA-induced megakaryocytic differentiation of K562 cells. C, immunoblot analysis of PDGFRA, JNK, and JUN levels; phosphorylated PDGFRA, JNK, and JUN levels; and JHEY1 and GATA-1 levels in the K562 cells transfected with miR-146b mimics or inhibitors or their controls and treated by hemin induction for 48 h. D, immunoblot analysis of PDGFRA, JNK, and JUN levels and phosphorylated PDGFRA, JNK, and JUN levels in the K562 cells transfected with miR-146b mimics or inhibitors or their controls and treated by PMA induction for 48 h. For all Western blots, GAPDH antibody was used to assess equal protein loading. E, immunoblot analysis of PDGFRA and GATA-1 levels in the K562 cells transfected with a construct carrying the PDGFRA ORF or an empty vector and with PDGFRA siRNAs or si-control and treated by hemin induction for 0, 24, and 48 h. F, immunoblot analysis of PDGFRA and GATA-1 levels in the K562 cells transfected with a construct carrying PDGFRA ORF or an empty vector and PDGFRA siRNAs or si-control and treated with PMA induction for 0, 24, and 48 h. For Western blots in E and F, GAPDH antibody was used to assess equal protein loading. The expression levels of PDGFRA and GATA-1 were calculated as a relative fold with respect to their expression in control-transfected cells before differentiation induction.