FIGURE 6.

ATP binding stimulates transthiolation reaction between Uba5 and Ufc1. Western blot image of Ufc1∼Ufm1 formation overtime. Ufc1 was added to pre-formed Uba5∼Ufm1 in the absence or presence of ATP, AMP-CPP, or AMP.

ATP binding stimulates transthiolation reaction between Uba5 and Ufc1. Western blot image of Ufc1∼Ufm1 formation overtime. Ufc1 was added to pre-formed Uba5∼Ufm1 in the absence or presence of ATP, AMP-CPP, or AMP.