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. 2014 Jun 25;289(33):22648–22658. doi: 10.1074/jbc.M114.573972

FIGURE 6.

FIGURE 6.

ATP binding stimulates transthiolation reaction between Uba5 and Ufc1. Western blot image of Ufc1∼Ufm1 formation overtime. Ufc1 was added to pre-formed Uba5∼Ufm1 in the absence or presence of ATP, AMP-CPP, or AMP.