TABLE 2.
Induction of inflammatory cytokines from PBMCs by KV261-Fc fusion protein
Inflammatory cytokines IFNγ, IL-1β, and TNFα were measured in human PBMC cultures after overnight treatment with various Kv1.3 blockers (all 2 μm) including KV261-Fc, KV261-HSA-34, KV261-HSA-686, and KV261 and controls including IgG4 Fc and HSA and no treatment. Cytokine measurements were performed as described under “Experimental Procedures,” and values are shown as averages of duplicates ± S.E. Values of <10.0 indicate cytokine levels were below the 10 pg/ml limit of detection.
| Blocker ID | Description | Cytokines (pg/ml) |
||
|---|---|---|---|---|
| IFNγ | IL-1β | TNFα | ||
| KV261-Fc | 261-GS(G4S)4-Fc | 236.3 ± 35.4 | 7202.6 ± 432.7 | 11104.8 ± 304.3 |
| KV261-HSA-34 | 261-AS(AP)20GS-HSA | <10.0 | 33.5 ± 22.4 | 57.4 ± 41.7 |
| KV261-HSA-686 | 261-AHRH-AS(AP)20GS-HSA | <10.0 | 71 ± 1.3 | 169 ± 38.4 |
| KV261 | 261 peptide | <10.0 | 26.9 ± 1.6 | 41.7 ± 2.8 |
| IgG4 Fc | Recombinant human Fc | <10.0 | 44.9 ± 1.3 | 140.8 ± 35.6 |
| HSA | Recombinant human albumin | <10.0 | 71.8 ± 22.4 | 144 ± 10.3 |
| No treatment | Buffer | <10.0 | 42.1 ± 0.4 | 144.4 ± 27.2 |