FIGURE 3.

USP20 depletion decreases Rad17 protein levels in a catalytic domain-dependent manner. A, effects of USP20 depletion on Rad17 levels in U2OS osteosarcoma line. B, similar phenotype of USP20 depletion in A549 lung cancer line. C, USP20 depletion results in Rad17 instability in normal human fibroblasts particularly after UV exposure. D, wild type but not catalytically-inactive, and siRNA resistant, USP20 rescues the decrease in Rad17 protein levels. EV, empty vector. Mut, catalytically mutant.