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Increased sensitivity to DNA damage after USP20 depletion. A, enhanced sensitivity of U2OS cells to cisplatin after using USP20 siRNA. Cells were exposed to the specified doses of cisplatin 72 h after the addition of siRNA, and viability was checked 48 h later by MTT assay as described under “Experimental Procedures.” B, similar phenotype in A549 cells. C, increased UV sensitivity after USP20 depletion in U2OS, and A549 (D) cancer cells.
