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. 2014 Jul 1;289(33):22865–22876. doi: 10.1074/jbc.M114.589473

FIGURE 6.

FIGURE 6.

Functionally silent nucleotides as modulators of the effects of “functionally active” nucleotides in CBS-PPases. A, ADP reversal of ATP activation of cnPPase and AMP reversal of ATP activation of elPPase. The fixed ATP concentration was 500 and 10 μm in experiments with elPPase and cnPPase, respectively. B, ADP inhibition of elPPase in the presence of 100 μm AMP. The dependence without AMP (dashed line) was taken from Fig. 5. C, AMP inhibition of elPPase in the presence of 20 μm ADP. The reaction medium contained 50 μm MgPPi and 5 mm Mg2+ in all cases. Activities without any nucleotide were taken as 100%. The lines show the best fits to Equation 2. rel., relative.