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. 2014 Jul 3;289(33):22991–23003. doi: 10.1074/jbc.M114.565044

FIGURE 3.

FIGURE 3.

Active site and secondary binding sites in CrISA1. A, substrate binding cleft of CrISA1 (monomer A) with bound maltoheptaose (orange) and glucose molecule (yellow). Selected substrate binding residues are displayed as green sticks. Glucosyl moieties of M7 are labeled G1 to G7 in blue and putative substrate binding subsites are labeled in red. B, zoom-in of the CrISA1 active site displaying the covalent bond between the G1 moiety of maltoheptaose and Asp-452. The contour map displayed in A and B is the Fo − Fc omit map, with maltoheptaose and the Asp-452 side chain omitted from map calculation, contoured at 3.0 σ and 5.0 σ, respectively. C, SBS1, and D, SBS2 carbohydrate binding sites. Fo − Fc omit maps surrounding the maltotriose moieties (pink) are contoured at 3.0 σ.