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. 2014 Jul 3;289(33):22991–23003. doi: 10.1074/jbc.M114.565044

TABLE 3.

UPLC elution conditions for the separation of 2-aminobenzamide-labeled maltooligosaccharides

Time (min) Flow rate % Solvent A (10 mm NH4CHOO buffer, pH 4.5) % Solvent B (MeCN)
ml/min
0 0.2 22 78
5 0.2 22 78
30 0.2 50 50
45 0.1 65 35
48 0.1 90 10
51 0.1 22 78
55 0.2 22 78